γ-Heregulin has no biological significance in primary breast cancer

British Journal of Cancer (2002) 86, 1362–1363. DOI: 10.1038/sj/bjc/6600245 www.bjcancer.co

Mutations and deletions of the SUZ12 polycomb gene in myeloproliferative neoplasms

International audienceNo abstract availabl

Sale conditions, Use and Consumption of provitamin A rich-foods in Douala-Cameroon: Case of crude palm oil

In order to contribute in fighting against vitamin A deficiency (VAD) in Cameroon through carefully use of crude palm oil (CPO), three surveys were carried out in Douala town and some of its surrounding rural areas. The first consisted of information collection from 55 sellers on the CPO's sales conditions (containers, exhibition conditions and liquefaction method). The second and the last was to collect information respectively on the use and the consumption of dishes prepared with unbleached CPO respectively from 199 housewives and 309 individuals. The results reveal that 87% of sellers exposed CPO in the open air during commercialization although 44% of them knew that sunlight could have a negative impact on its quality. Heating and/or sun exposure were the technics used to liquefy oil. On the 98% of households that prepared with CPO, 93% of them often bleached it, although 64% were aware of the detrimental effect of this process. The social status of housewives significantly influenced this result. Unbleached CPO was used to prepare many dishes which were frequently consumed by 87% of people with a significant influence of lifestyle and region of origin. The results of this study showed that CPO could be used for fight against VAD in Cameroon if it is carefully commercialized and used in households and if people frequently consume dishes prepared with unbleached CPO. Practical application The knowledge of information on selling conditions, use and consumption of CPO will contribute to fight against VAD. In fact, it will guide policies for the development of strategies to improve in its commercialization conditions; to propagate the CPO and its consumption throughout the country and to improve nutritional education

Multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) to identify recurrent chromosomal alterations in breast tumour cell lines

Recurrent chromosome breakpoints in tumour cells may point to cancer genes, but not many have been molecularly characterised. We have used multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) on breast tumour cell lines to identify regions of chromosome break created by inversions, duplications, insertions and translocations on chromosomes 1, 5, 8, 12 and 17. We delineate a total of 136 regions of break, some of them occurring with high frequency. We further describe two examples of dual-colour FISH characterisation of breakpoints, which target the 1p36 and 5p11–12 regions. Both breaks involve genes whose function is unknown to date. The mbanding-FISH strategy constitutes an efficient first step in the search for potential cancer genes

Probing the faint end Luminosity Function of Lyman Alpha Emitters at 3<z<7 behind 17 MUSE lensing clusters

We present a study of the galaxy Lyman-alpha luminosity function (LF) using a sample of 17 lensing clusters observed by the MUSE/VLT. Magnification from strong gravitational lensing by clusters of galaxies and MUSE apabilities allow us to blindly detect LAEs without any photometric pre-selection, reaching the faint luminosity regime. 600 lensed LAEs were selected behind these clusters in the redshift range 2.9<$z$< 6.7, covering four orders of magnitude in magnification-corrected Lyman-alpha luminosity (39.0<log$L$< 43.0). The method used in this work ($V_{\text{max}}$) follows the recipes originally developed by arXiv:1905.13696(N) (DLV19) with some improvements to better account for the effects of lensing when computing the effective volume. The total co-moving volume at 2.9<$z$<6.7 is $\sim$50 $10^{3}Mpc^{3}$. Our LF points in the bright end (log L)>42 are consistent with those obtained from blank field observations. In the faint luminosity regime, the density of sources is well described by a steep slope, $\alpha\sim-2$ for the global redshift range. Up to log(L)$\sim$41, the steepening of the faint end slope with redshift, suggested by the earlier work of DLV19 is observed, but the uncertainties remain large. A significant flattening is observed towards the faintest end, for the highest redshift bins (log$L$<41). Using face values, the steep slope at the faint-end causes the SFRD to dramatically increase with redshift, implying that LAEs could play a major role in the process of cosmic reionization. The flattening observed towards the faint end for the highest redshift bins still needs further investigation. This turnover is similar to the one observed for the UV LF at $z\geq6$ in lensing clusters, with the same conclusions regarding the reliability of current results (e.g.arXiv:1803.09747(N); arXiv:2205.11526(N)).Comment: 20 pages, 15 figures, 6 tables. Accepted for publication in A\&

Genome profiling of ERBB2-amplified breast cancers

<p>Abstract</p> <p>Background</p> <p>Around 20% of breast cancers (BC) show <it>ERBB2 </it>gene amplification and overexpression of the ERBB2 tyrosine kinase receptor. They are associated with a poor prognosis but can benefit from targeted therapy. A better knowledge of these BCs, genomically and biologically heterogeneous, may help understand their behavior and design new therapeutic strategies.</p> <p>Methods</p> <p>We defined the high resolution genome and gene expression profiles of 54 <it>ERBB2</it>-amplified BCs using 244K oligonucleotide array-comparative genomic hybridization and whole-genome DNA microarrays. Expression of ERBB2, phosphorylated ERBB2, EGFR, IGF1R and FOXA1 proteins was assessed by immunohistochemistry to evaluate the functional ERBB2 status and identify co-expressions.</p> <p>Results</p> <p>First, we identified the <it>ERBB2</it>-<it>C17orf37</it>-<it>GRB7 </it>genomic segment as the minimal common 17q12-q21 amplicon, and <it>CRKRS </it>and <it>IKZF3 </it>as the most frequent centromeric and telomeric amplicon borders, respectively. Second, GISTIC analysis identified 17 other genome regions affected by copy number aberration (CNA) (amplifications, gains, losses). The expression of 37 genes of these regions was deregulated. Third, two types of heterogeneity were observed in <it>ERBB2</it>-amplified BCs. The genomic profiles of estrogen receptor-postive (ER+) and negative (ER-) <it>ERBB2</it>-amplified BCs were different. The WNT/β-catenin signaling pathway was involved in ER- <it>ERBB2</it>-amplified BCs, and <it>PVT1 </it>and <it>TRPS1 </it>were candidate oncogenes associated with ER+ <it>ERBB2</it>-amplified BCs. The size of the <it>ERBB2 </it>amplicon was different in inflammatory (IBC) and non-inflammatory BCs. <it>ERBB2</it>-amplified IBCs were characterized by the downregulated and upregulated mRNA expression of ten and two genes in proportion to CNA, respectively. IHC results showed (i) a linear relationship between <it>ERBB2 </it>gene amplification and its gene and protein expressions with a good correlation between ERBB2 expression and phosphorylation status; (ii) a potential signaling cross-talk between EGFR or IGF1R and ERBB2, which could influence response of <it>ERBB2</it>-positive BCs to inhibitors. FOXA1 was frequently coexpressed with ERBB2 but its expression did not impact on the outcome of patients with <it>ERBB2</it>-amplified tumors.</p> <p>Conclusion</p> <p>We have shown that ER+ and ER- <it>ERBB2</it>-amplified BCs are different, distinguished <it>ERBB2 </it>amplicons in IBC and non-IBC, and identified genomic features that may be useful in the design of alternative therapeutical strategies.</p

Pilot-WINGS: An extended MUSE view of the structure of Abell 370

We investigate the strong-lensing cluster Abell 370 (A370) using a wide Integral Field Unit (IFU) spectroscopic mosaic from the Multi-Unit Spectroscopic Explorer (MUSE). IFU spectroscopy provides significant insight into the structure and mass content of galaxy clusters, yet IFU-based cluster studies focus almost exclusively on the central Einstein-radius region. Covering over 14 arcmin2, the new MUSE mosaic extends significantly beyond the A370 Einstein radius, providing, for the first time, a detailed look at the cluster outskirts. Combining these data with wide-field, multi-band Hubble Space Telescope (HST) imaging from the BUFFALO project, we analyse the distribution of objects within the cluster and along the line of sight. Identifying 416 cluster galaxies, we use kinematics to trace the radial mass profile of the halo, providing a mass estimate independent from the lens model. We also measure radially averaged properties of the cluster members, tracking their evolution as a function of infall. Thanks to the high spatial resolution of our data, we identify six cluster members acting as galaxy–galaxy lenses, which constrain localized mass distributions beyond the Einstein radius. Finally, taking advantage of MUSE’s 3D capabilities, we detect and analyse multiple spatially extended overdensities outside of the cluster that influence lensing-derived halo mass estimates. We stress that much of this work is only possible thanks to the robust, extended IFU coverage, highlighting its importance even in less optically dense cluster regions. Overall, this work showcases the power of combining HST + MUSE, and serves as the initial step towards a larger and wider program targeting several clusters

Nodular Worm Infection in Wild Chimpanzees in Western Uganda: A Risk for Human Health?

This study focused on Oeosophagostomum sp., and more especially on O. bifurcum, as a parasite that can be lethal to humans and is widespread among humans and monkeys in endemic regions, but has not yet been documented in apes. Its epidemiology and the role played by non-human primates in its transmission are still poorly understood. O. stephanostomum was the only species diagnosed so far in chimpanzees. Until recently, O. bifurcum was assumed to have a high zoonotic potential, but recent findings tend to demonstrate that O. bifurcum of non-human primates and humans might be genetically distinct. As the closest relative to human beings, and a species living in spatial proximity to humans in the field site studied, Pan troglodytes is thus an interesting host to investigate. Recently, a role for chimpanzees in the emergence of HIV and malaria in humans has been documented. In the framework of our long-term health monitoring of wild chimpanzees from Kibale National Park in Western Uganda, we analysed 311 samples of faeces. Coproscopy revealed that high-ranking males are more infected than other individuals. These chimpanzees are also the more frequent crop-raiders. Results from PCR assays conducted on larvae and dried faeces also revealed that O. stephanostomum as well as O. bifurcum are infecting chimpanzees, both species co-existing in the same individuals. Because contacts between humans and great apes are increasing with ecotourism and forest fragmentation in areas of high population density, this paper emphasizes that the presence of potential zoonotic parasites should be viewed as a major concern for public health. Investigations of the parasite status of people living around the park or working inside as well as sympatric non-human primates should be planned, and further research might reveal this as a promising aspect of efforts to reinforce measures against crop-raiding

Impact of environmental radiation on the health and reproductive status of fish from Chernobyl

© 2018 American Chemical Society. Aquatic organisms at Chernobyl have now been chronically exposed to environmental radiation for three decades. The biological effects of acute exposure to radiation are relatively well documented, but much less is known about the long-term effects of chronic exposure of organisms in their natural environment. Highly exposed fish in freshwater systems at Chernobyl showed morphological changes in their reproductive system in the years after the accident. However, the relatively limited scope of past studies did not allow robust conclusions to be drawn. Moreover, the level of the radiation dose at which significant effects on wildlife occur is still under debate. In the most comprehensive evaluation of the effects of chronic radiation on wild fish populations to date, the present study measures specific activities of 137Cs, 90Sr, and transuranium elements (238Pu, 239,240Pu, and 241Am), index conditions, distribution and size of oocytes, as well as environmental and biological confounding factors in two fish species perch (Perca fluviatilis) and roach (Rutilus rutilus) from seven lakes. In addition, relative species abundance was examined. The results showed that both fish species are, perhaps surprisingly, in good general physiological and reproductive health. Perch, however, appeared to be more sensitive to radiation than roach: in the most contaminated lakes, a delay of the maturation of the gonads and the presence of several undeveloped phenotypes were evident only for perch and not for roach